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Human retroviruses : methods and protocols / edited by Elisa Vicenzi and Guido Poli.

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Language:
English.
Publication date:
2014
Copyright date:
2014
Publication:
New York : Humana Press, [2014].
Copyright notice:
©2014
Format:
  • Book
  • xviii, 345 pages : illustrations (some colored) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Part I. Mapping the HIV Life Cycle
  • Virion Attachment and Entry: HIV gp120 Env Biotinylation, gp120 Env, or Integrin Ligand-Binding Assay / Claudia Cicala and James Arthos
  • CryoEM Analysis of Capsid Assembly and Structural Changes Upon Interactions with a Host Restriction Factor, TRIM5[alpha] / Gongpu Zhao and Peijun Zhang
  • The Fate of HIV-1 Capsid: A Biochemical Assay for HIV-1 Uncoating / Yang Yang, Jeremy Luban, and Felipe Diaz-Griffero
  • The Cyclosporin A Washout Assay to Detect HIV-1 Uncoating in Infected Cells / Amy E. Hulme and Thomas J. Hope
  • Imaging HIV-1 Nuclear Pre-integration Complexes / Anna Cereseto and Mauro Giacca
  • HIV-1 Reverse Transcription / Andrea Cimarelli and Jean-Luc Darlix
  • RNase H: Specificity, Mechanisms of Action, and Antiviral Target / Karin Moelling, Felix Broecker, and John E. Kerrigan
  • HIV-1 Chromatin, Transcription, and the Regulatory Protein Tat / Laurence Colin, Eric Verdin, and Carine Van Lint
  • HIV-1 Rev Function and RNA Nuclear-Cytoplasmic Export / Alan Cochrane
  • HIV-1 Accessory Proteins: Nef / Anke Heigele, Daniel Sauter, Jan Münch, and Frank Kirchhoff
  • HIV-1 Accessory Proteins: VpR / Richard Y. Zhao and Michael I. Bukrinsky
  • HIV-1 Accessory Proteins: Vpu and Vif / Amy Andrew and Klaus Strebel
  • SIVSM/HIV-2 Vpx Proteins: Function and Uses in the Infection of Primary Myeloid Cells / Gregory Berger and Andrea Cimarelli
  • Imaging of HIV Assembly and Release / Barbara Müller and Jacomine Krijnse-Locker.
  • Part II. Isolation, Co-Receptor Use, and Cell Tropism of HIV-1
  • HIV-1 Isolation from Infected Peripheral Blood Mononuclear Cells / Stefania Dispinseri, Elisa Saba, Elisa Vicenzi, Neeltje A. Kootstra, Hanneke Schuitemaker, and Gabriella Scarlatti
  • Determination of HIV-1 Co-receptor Usage / Mariangela Cavarelli and Gabriella Scarlatti
  • The Macrophage and HIV: Basic Concepts and Methodologies / Suzanne Gartner
  • HIV Infection of Dendritic Cells / Najla Nasr, Andrew Harman, Stuart Turville, and Anthony L. Cunningham
  • Histocultures (Tissue Explants) in Human Retrovirology / Anush Arakelyan, Wendy Fitzgerald, Jean-Charles Grivel, Christophe Vanpouille, and Leonid Margolis.
  • Part III. In Vivo Quantification of HIV-1
  • Single-Copy Quantification of HIV-1 in Clinical Samples / Ann Wiegand and Frank Maldarelli
  • Quantification of Total HIV1-DNA in Peripheral Blood Mononuclear Cells / Christine Rouzioux, Adeline Mélard, and Véronique Avéttand-Fénoël.
  • Part IV. Biotechnological Aspects of HIV-1
  • HIV-1-Based Lentiviral Vectors / Ying Poi Liu and Ben Berkhout
  • Quantification of miRNA by Poly(A)-RT-qPCR Arrays and Verification of Target Sites in HIV-1 Using a One-LTR Infectious Molecular Clone / Zachary A. Klase, Laurent Houzet, and Kuan-Teh Jeang.
  • Part V. HTLVS
  • Investigating Human T Cell Lymphotropic Retrovirus (HTLV) Tax Function with Molecular and Immunophenotypic Techniques / Greta Forlani, Roberto S. Accolla, and Giovanna Tosi
  • Proviral Load Determination of HTLV-1 and HTLV-2 in Patients' Peripheral Blood Mononuclear Cells by Real-Time PCR / Claudio Casoli, Elisabetta Pilotti, and Umberto Bertazzoni
  • Quantitative Analysis of Human T-Lymphotropic Virus Type 1 (HTLV-1) Gene Expression Using Nucleo-Cytoplasmic Fractionation and Splice Junction-Specific Real-Time RT-PCR (qRT-PCR) / Ilaria Cavallari, Francesca Rende, and Vincenzo Ciminale.
Contributor:
Vicenzi, Elisa, editor.
Poli, Guido, editor.
Springer Science+Business Media, copyright holder.
Series:
Methods in molecular biology, 1064-3745 ; 1087
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; 1087.
Springer protocols (Series)
Subjects:
ISBN:
1627036695
9781627036696

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