Problem solving in enzyme biocatalysis
 Author/Creator
 Illanes, Andrés, author.
 Language
 English.
 Edition
 First edition.
 Publication
 Chichester, West Sussex, United Kingdom : Wiley, 2014.
 Physical description
 1 online resource (xxvi, 318 pages)
Access
Available online
More options
Contributors
 Contributor
 Wilson, Lorena, author.
 Vera, Carlos, author.
Contents/Summary
 Contents

 Preface ix Nomenclature xi Epsilon Software Information xxi 1 Facts and Figures in Enzyme Biocatalysis 1 1.1 Introduction 1 1.2 Enzymes as Process Catalysts 3 1.3 Evolution of Enzyme Biocatalysis: From Hydrolysis toSynthesis 5 1.4 The Enzyme Market: Figures and Outlook 6 References 7 2 Enzyme Kinetics in a Homogeneous System 11 2.1 Introduction 11 2.2 Theory of Enzyme Kinetics 14 2.3 SingleSubstrate Reactions 17 2.4 MultipleSubstrate Reactions 19 2.5 MultipleEnzyme Reactions 21 2.6 Determination of Kinetic Parameters 22 2.7 Effects of Operational Variables on Enzyme Kinetics 24 Solved Problems 29 Supplementary Problems 72 References 84 3 Enzyme Kinetics in a Heterogeneous System 87 3.1 Introduction 87 3.2 Immobilization of Enzymes 87 3.3 MassTransfer Limitations in Enzyme Catalysis 92 3.4 Determination of Intrinsic Kinetic and MassTransferParameters 102 Solved Problems 105 Supplementary Problems 127 References 138 4 Enzyme Reactor Design and Operation under Ideal Conditions141 4.1 Modes of Operation and Reactor Configurations 141 4.2 Definition of Ideal Conditions 142 4.3 Strategy for Reactor Design and Performance Evaluation143 4.4 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Ideal Conditions 143 Solved Problems 157 Supplementary Problems 174 References 179 5 Enzyme Reactor Design and Operation under MassTransferLimitations 181 5.1 Sequential Batch and Continuously Operated Reactors withImmobilized Enzymes 182 5.2 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under MassTransfer Limitations 183 Solved Problems 185 Supplementary Problems 198 6 Enzyme Reactor Design and Operation under BiocatalystInactivation 203 6.1 Mechanistically Based Mathematical Models of EnzymeInactivation 203 6.2 Effect of Catalytic Modulators on Enzyme Inactivation205 6.3 Mathematical Models for Different Enzyme Kinetics, Modes ofOperation, and Reactor Configurations under BiocatalystInactivation 206 6.4 Mathematical Models for Enzyme Kinetics, Modes of Operation, and Reactor Configurations under Simultaneous MassTransferLimitations and Enzyme Inactivation 212 6.5 Strategies for Reactor Operation under BiocatalystInactivation 213 Solved Problems 215 Supplementary Problems 233 References 240 7 Optimization of Enzyme Reactor Operation 243 7.1 Strategy for the Optimization of Enzyme Reactor Performance244 7.2 Mathematical Programming for Static Optimization 247 7.3 Dynamic Programming 248 7.4 Statistical Optimization by Surface Response Methodology249 Solved Problems 254 Supplementary Problems 272 References 275 Appendix A Mathematical Methods 277 A.1. Newton s Method 277 A.2. Curve Fitting by Least Squares 280 A.3. Solving Ordinary Differential Equations 296 A.4. Numerical Methods for Solving Differential Equations302 References 310 Index 311.
 (source: Nielsen Book Data)
 Publisher's Summary
 Enzyme biocatalysis is a fastgrowing area in processbiotechnology that has expanded from the traditional fields offoods, detergents, and leather applications to more sophisticateduses in the pharmaceutical and finechemicals sectors andenvironmental management. Conventional applications of industrialenzymes are expected to grow, with major opportunities in thedetergent and animal feed sectors, and new uses in biofuelproduction and human and animal therapy. In order to design more efficient enzyme reactors and evaluateperformance properly, sound mathematical expressions must bedeveloped which consider enzyme kinetics, material balances, andeventual mass transfer limitations. With a focus on problemsolving, each chapter provides abridged coverage of the subject, followed by a number of solved problems illustrating resolutionprocedures and the main concepts underlying them, plussupplementary questions and answers. Based on more than 50 years of teaching experience, ProblemSolving in Enzyme Biocatalysis is a unique reference forstudents of chemical and biochemical engineering, as well asbiochemists and chemists dealing with bioprocesses. Contains: Enzyme properties and applications; enzyme kinetics; enzyme reactor design and operation 146 worked problems andsolutions in enzyme biocatalysis.
(source: Nielsen Book Data)
Subjects
Bibliographic information
 Publication date
 2014
 Responsibility
 Andrés Illanes, Lorena Wilson, Carlos Vera.
 Note
 Edition statement from running title area.
 Includes bibliographical references and index.
 Available in another form
 Print version: Illanes, Andrés. Problem solving in enzyme biocatalysis. Chichester, West Sussex, United Kingdom : Wiley, 2013 9781118341711 (DLC) 2013029486 (OCoLC)842883207
 ISBN
 9781118341742
 9781118341759
 1118341759
 1118341740