Identifying microbes by mass spectrometry proteomics
- Boca Raton : CRC Press, Taylor & Francis Group, 
- Copyright notice
- Physical description
- xviii, 271 pages : illustrations ; 24 cm
QP519.9 .M3 I346 2014
- Unknown QP519.9 .M3 I346 2014
- Wick, Charles Harold, editor.
- Includes bibliographical references (pages 239-262) and index.
- Detection and Identification of Microbes Using Mass Spectrometry Proteomics Charles H. Wick Mass Analyzers and MS/MS Methods for Microbial Detection and Identification Michael F. Stanford Matching Mass Spectral Profiles of Biomarkers Michael F. Stanford Sequence Information Derived from Proteins or Nucleic Acids Samir V. Deshpande Collection and Processing of Microbial Samples Samir V. Deshpande Computer Software Used for Chemometric and Bioinformatics to Discriminate Microbes Samir V. Deshpande Applications Charles H. Wick Survey of Commercially Available MS-Based Platforms Suitable for Bacterial Detection and Identification Michael F. Stanford Current and Future Trends in Using MS for Microbial Detection and Identification Charles H. Wick References Index.
- (source: Nielsen Book Data)
- Publisher's Summary
- All microbes, including bacteria, viruses, and fungi, can be classified and identified by matching a few peptides known to be unique to each organism. Identifying Microbes by Mass Spectrometry Proteomics describes ways to identify microorganisms using powerful new techniques combining hardware and software and yielding highly accurate methods for detection, identification, and classification of microbes. This straightforward technology can be used to detect unknown and unsequenced microorganisms as well as microbes in complex environmental samples. This book reviews various mass analyzers used for detection and describes ionization methods frequently used for analysis of microbial constituents, a necessary step in the preparation of mass spectrometry (MS) samples. The text also discusses diverse processing methods, which are used to analyze MS files for matching mass spectral profiles, and examines protein and nucleic acid sequence-based methods capable of classification and identification of microbial agents. The book also covers sample collection methods and specific sample preparation techniques. The text addresses using computer software and bioinformatics approaches for data mining to discriminate microbes using mass spectrometry proteomics (MSP). It also discusses historical pattern recognition-based methods and other approaches such as analysis of pyrolysis products, chemical ionization (CI) of fatty acid methyl esters, and MALDI-MS. The text contains examples of the application of the MSP technique for microbe detection and includes a survey of suitable and commercially available MS-based platforms. Successful applications include the identification of unknown microbes in honey bees associated with colony collapse disorder and the analysis of virus strains from the 2009 influenza pandemic. The final chapter outlines future trends in these groundbreaking uses of MS techniques, which are fast, not limited by sample type, and show potential in answering complex environmental questions.
(source: Nielsen Book Data)
- Publication date
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- edited by Charles H. Wick.