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Pluripotent stem cells : methods and protocols / edited by Uma Lakshmipathy, Mohan C. Vemuri.

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Language:
English.
Publication date:
2013
Imprint:
New York : Humana Press : Springer, c2013.
Format:
  • Book
  • x, 292 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index. Sources and derivation of human embryonic stem cells / Michal Amit -- New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani -- Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao -- Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore -- Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu ... [et al.] -- Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions / Andrew Fontes ... [et al.] -- Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers -- Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins -- Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur ... [et al.] -- Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku -- Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie -- Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis -- Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin -- Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr. -- TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel ... [et al.] -- Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart -- Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete -- Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu -- Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner -- Cloning technologies / Andrew Fontes -- Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy -- Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology / Chad C. MacArthur.
Contents:
  • Sources and derivation of human embryonic stem cells / Michal Amit
  • New chemical approach to the efficient generation of mouse embryonic stem cells / Hossein Baharvand and Seyedeh-Nafiseh Hassani
  • Review of the methods for human iPSC derivation / Nasir Malik and Mahendra S. Rao
  • Generation of human-induced pluripotent stem cells by lentiviral transduction / Jennifer C. Moore
  • Generation of induced pluripotent stem cells with CytoTune, a non-integrating sendai virus / Pauline T. Lieu ... [et al.]
  • Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined essential 8TM medium conditions / Andrew Fontes ... [et al.]
  • Feeder-free substrates for pluripotent stem cell culture / Alexandria Sams and Mark J. Powers
  • Methods for culturing human embryonic stem cells on feeders / Jasmeet Kaur and Mary Lynn Tilkins
  • Methods for culturing human embryonic stem cells in a xeno-free system / Jasmeet Kaur ... [et al.]
  • Directed differentiation of human pluripotent stem cells along the pancreatic endocrine lineage / Dennis Van Hoof and Muluye E. Liku
  • Directed differentiation of pluripotent stem cells to functional hepatocytes / Philip Roelandt, Jolien Vanhove, and Catherine Verfaillie
  • Highly efficient directed differentiation of human induced pluripotent stem cells into cardiomyocytes / Paul W. Burridge and Elias T. Zambidis
  • Generation of transgene-free iPSC lines from human normal and neoplastic blood cells using episomal vectors / Kejin Hu and Igor Slukvin
  • Cellular characterization of human pluripotent stem cells / Rene H. Quintanilla Jr.
  • TaqMan® OpenArray® high-throughput transcriptional analysis of human embryonic and induced pluripotent stem cells / Sunali N. Patel ... [et al.]
  • Epigenome analysis of pluripotent stem cells / Christopher L. Ricupero, Mavis R. Swerdel, and Ronald P. Hart
  • Single cell gene expression analysis of pluripotent stem cells / Ronald V. Abruzzese and Richard A. Fekete
  • Profiling stem cells using quantitative PCR protein assays / David Ruff and Pauline T. Lieu
  • Labeled stem cells as disease models and in drug discovery / Catharina Ellerström, Raimund Strehl, and Johan Hyllner
  • Cloning technologies / Andrew Fontes
  • Stable transfection using episomal vectors to create modified human embryonic stem cells / Ying Liu, Kate Judd, and Uma Lakshmipathy
  • Site-specific integration in human ESC using Jump-In (TM) TI(TM) technology / Chad C. MacArthur.
Contributor:
Lakshmipathy, Uma.
Vemuri, Mohan C.
Series:
Methods in molecular biology, 1064-3745 ; 997
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 997.
Springer protocols (Series)
Subjects:
ISBN:
1627033475
9781627033473

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