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Ion channels : methods and protocols / edited by Nikita Gamper.

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Language:
English.
Publication date:
2013
Imprint:
2nd ed. - New York : Humana Press : Springer, c2013.
Format:
  • Book
  • xiv, 440 p. : ill. (some col.) ; 26 cm.
Note:
Previous ed.: 2006.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Approaches to cloning of pain-related ion channel genes / Armen N. Akopian
  • Mammalian expression systems and transfection techniques / Daunia Laurenti and Lezanne Ooi
  • Use of Escherichia coli for the production and purification of membrane proteins / Vincent G.L. Postis ... [et al.]
  • Transient overexpression of genes in neurons using nucleofection / Hannah M. Kirton, Louisa Pettinger, and Nikita Gamper
  • Viral gene delivery : optimized protocol for production of high titer lentiviral vectors / James Hewinson, Julian F.R. Paton, and Sergey Kasparov
  • Two-electrode voltage clamp / Bingcai Guan, Xingjuan Chen, and Hailin Zhang
  • Conventional micropipette-based patch clamp techniques / Jonathan D. Lippiat and David C. Wrighton
  • Recording of ion channel activity in planar lipid bilayer experiments / Eleonora Zakharian
  • Recording macroscopic currents in large patches from Xenopus oocytes / Tibor Rohacs
  • Combined single-channel and macroscopic recording techniques to analyze gating mechanisms of the large conductance Ca²⁺ and voltage activated (BK) potassium channel / Nguyen V. Nguyen ... [et al.]
  • Perforated whole-cell patch-clamp recording / John E. Linley
  • Piezo-electrically driven mechanical stimulation of sensory neurons / Jizhe Hao ... [et al.]
  • Automated planar patch-clamp / Carol J. Milligan and Clemens Möller
  • Recording single-channel currents using "smart patch-clamp" technique / Anamika Bhargava and Julia Gorelik
  • Using total internal reflection fluorescence microscopy to observe ion channel trafficking and assembly / Sarah Schwarzer ... [et al.]
  • Förster resonance energy transfer-based imaging at the cell surface of live cells / Sonya M. Bierbower and Mark S. Shapiro
  • Use of dansyl-calmodulin to study interactions with channels and other proteins / Alessandro Alaimo ... [et al.]
  • Imaging and quantification of recycled KATP channels / Christopher J. Cockcroft
  • Generation of antibodies that are externally acting isoform-specific inhibitors of ion channels / Jacqueline Naylor and David J. Beech
  • Site-directed mutagenesis to study the structure-function relationships of ion channels / Wei Yang and Lin-Hua Jiang
  • Cysteine-based cross-linking approach to study inter-domain interactions in ion channels / Lin-Hua Jiang
  • Analysis of Ca²⁺⁻ binding sites in the MthK RCK domain by x-ray crystallography / Frank J. Smith and Brad S. Rothberg
  • Isotope labeling strategies for analysis of an ion channel cytoplasmic domain by NMR spectroscopy / Karin Abarca-Heidemann ... [et al.]
  • Recording dendritic ion channel properties and function from cortical neurons / Mala M. Shah
  • M-current recording from acute DRG slices / Kirstin E. Rose, Sylvain Gigout, and Nikita Gamper
  • Studying ion channels in human erythrocytes by direct and indirect means / Stephan M. Huber
  • Recording ion channels in isolated, split-opened tubules / Elena Mironova ... [et al.]
  • Single-channel analysis of TRPC channels in the podocytes of freshly isolated glomeruli / Daria V. Ilatovskaya and Alexander Staruschenko
  • Ca 2 + imaging as a tool to assess TRP channel function in murine distal nephrons / Mykola Mamenko ... [et al.]
  • Patch-clamping Drosophila sensory neurons / Volodymyr Kucher ... [et al.]
  • Production and validation of recombinant adeno-associated virus for channelrhodopsin expression in neurons / John Y. Lin
  • Optical control of ligand-gated ion channels / Stephanie Szobota, Catherine McKenzie, and Harald Janovjak.
Contributor:
Gamper, Nikita.
Series:
Methods in molecular biology, 1064-3745 ; 998
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; 998.
Springer protocols (Series)
Subjects:
ISBN:
1627033505
9781627033503

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