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Target identification and validation in drug discovery : methods and protocols / edited by Jürgen Moll, Riccardo Colombo.



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Publication date:
New York : Humana Press : Springer, c2013.
  • Book
  • xv, 371 p. : ill. (some col.) ; 26 cm.
Includes bibliographical references and index.
  • The path to oncology drug target validation : an industry perspective
  • Identification of aptamers as specific binders and modulators of cell-surface receptor activity
  • The design and structure-functional properties of DNA-based immunomodulatory sequences
  • SiRNA design principles and off-target effects
  • Western blot evaluation of siRNA delivery by ph-responsive peptides
  • High-throughput RNAi screening for the identification of novel targets
  • Integration of RNAi and small molecule screens to identify targets for drug development
  • CellProfiler and KNIME : Open source tools for high content screening
  • PARP inhibition as a prototype for synthetic lethal screens
  • Structure-based target druggability assessment
  • Validating pharmacological disruption of protein-protein interactions by acceptor photobleaching FRET imaging
  • Systematic analysis of complex signal transduction pathways using protein fragment complementation assays
  • Reverse phase protein microarrays and their utility in drug development
  • A cell culture system that mimics chronic lymphocytic leukemia cells microenvironment for drug screening and characterization
  • Two-dimensional vs. Three-dimensional in vitro tumor migration and invasion assays
  • Tumor spheroid-based migration assays for evaluation of therapeutic agents
  • The neurosphere assay applied to neural stem cells and cancer stem cells
  • Genetically engineered animal models for in vivo target identification and validation in oncology
  • Target validation in mice by constitutive and conditional RNAi
  • In vivo target validation by inducible RNAi in human xenograft mouse models
  • Bright-field in situ hybridization methods to discover gene amplifications and rearrangements in clinical samples
  • Combined microRNA in situ hybridization and immunohistochemical detection of protein markers.
Moll, Jürgen.
Colombo, Riccardo.
Methods in molecular biology, 1940-6029 ; v. 986
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; v. 986.
Springer protocols (Series)

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