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Cytochrome P450 protocols / edited by Ian R. Phillips, Elizabeth A. Shephard, Paul R. Ortiz de Montellano.

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Language:
English.
Publication date:
2013
Imprint:
3rd ed. - New Jersey : Humana Press, c2013.
Format:
  • Book
  • xii, 319 p. : ill. (some col.) ; 27 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Bioluminescent assays for cytochrome P450 enzymes / Douglas S. Auld, Henrike Veith, and James J. Cali
  • Simultaneous determination of multiple CYP inhibition constants using a cocktail-probe approach / Michael Zientek and Kuresh Youdim
  • High-throughput mass spectrometric cytochrome P450 inhibition screening / Kheng B. Lim, Can C. Ozbal, and Daniel B. Kassel
  • Synthesis, characterization, and application of ¹³c-methyl isocyanide as an NMR probe of heme protein active sites / Christopher McCullough ... [et al.]
  • High-throughput fluorescence assay for cytochrome P450 mechanism-based Inactivators / Cesar Kenaan, Haoming Zhang, and Paul F. Hollenberg
  • Identification of endogenous substrates of orphan cytochrome P450 enzymes through the use of untargeted metabolomics approaches / Qian Cheng and F. Peter Guengerich
  • Genetic and mass spectrometric tools for elucidating the physiological function(s) of cytochrome P450 enzymes from Mycobacterium tuberculosis / Hugues Ouellet ... [et al.]
  • Escherichia coli expression-based approach for porphyrin substitution in heme proteins / Michael B. Winter, Joshua J. Woodward, and Michael A. Marletta
  • Expression in Escherichia coli of a cytochrome P450 enzyme with a cobalt protoporphyrin IX porosthetic group / Wesley E. Straub ... [et al.]
  • Nanodiscs in the studies of membrane-bound cytochrome P450 enzymes / A. Luthra ... [et al.]
  • Rapid LC-MS drug metabolite profiling using bioreactor particles / Linlin Zhao, Besnik Bajrami, and James F. Rusling
  • Fluorescence-based screening of cytochrome P450 activities in intact cells / M. Teresa Donato and M. José Gómez-Lechón
  • Screening for cytochrome P450 reactivity with a reporter enzyme / Kersten S. Rabe and Christof M. Niemeyer
  • High-throughput fluorescence assay of cytochrome P450 3A4 / Qian Cheng and F. Peter Guengerich
  • Targeted protein capture for analysis of electrophile-protein adducts / Rebecca E. Connor ... [et al.]
  • DNA shuffling of cytochrome P450 enzymes / James B.Y.H. Behrendorff, Wayne A. Johnston, and Elizabeth M.J. Gillam
  • Measurement of P450 difference spectra using intact cells / Wayne A. Johnston and Elizabeth M.J. Gillam
  • DNA shuffling of cytochromes P450 for indigoid pigment production / Nedeljka N. Rosic
  • P450 oxidoreductase : genotyping, expression, purification of recombinant protein, and activity assessments of wild-type and mutant protein / Vishal Agrawal and Walter L. Miller
  • LICRED : a versatile drop-in vector for rapid generation of redox-self-sufficient cytochromes P450 / Federico Sabbadin, Gideon Grogan, and Neil C. Bruce
  • Update on allele nomenclature for human cytochromes P450 and the human cytochrome P450 allele (CYP-allele) nomenclature database / Sarah C. Sim and Magnus Ingelman-Sundberg
  • Simultaneous in vivo phenotyping of CYP enzymes / Sussan Ghassabian and Michael Murray
  • Detection of regulatory polymorphisms : high-throughput capillary DNase I footprinting / Matthew Hancock and Elizabeth A. Shephard
  • Isolation of mouse hepatocytes / Mina Edwards ... [et al.]
  • Highly efficient SiRNA and gene transfer into hepatocyte-like HepaRG cells and primary human hepatocytes : new means for drug metabolism and toxicity studies / Véronique Laurent ... [et al.].
Contributor:
Phillips, Ian R. (Ian Randall), 1951-
Shephard, Elizabeth A. (Elizabeth Anne), 1950-
Ortiz de Montellano, Paul R.
Series:
Methods in molecular biology, 1064-3745 ; 987
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; v. 987.
Springer protocols (Series)
Subjects:
ISBN:
9781627033206
1627033203

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