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Enzyme engineering : methods and protocols / edited by James C. Samuelson.



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Publication date:
New York : Humana Press : Springer, c2013.
  • Book
  • xi, 252 p. : ill. (some col.) ; 27 cm.
Includes bibliographical references and index.
  • Tripartite fusion system for the selection of protein variants with increased stability in vivo / Linda Foit and James C.A. Bardwell
  • Determining enzyme kinetics via isothermal titration calorimetry / Neil A. Demarse ... [et al.]
  • GFP reporter screens for the engineering of amino acid degrading enzymes from libraries expressed in bacteria / Olga Paley ... [et al.]
  • Flow cytometric assays for interrogating LAGLIDADG homing endonuclease DNA-binding and cleavage properties / Sarah K. Baxter ... [et al.]
  • TAL effector nuclease (TALEN) engineering / Ting Li and Bing Yang
  • In vitro evolution of enzymes / Misha V. Golynskiy ... [et al.]
  • Residue-specific incorporation of unnatural amino acids into proteins in vitro and in vivo / Amrita Singh-Blom, Randall A. Hughes, and Andrew D. Ellington
  • Reconstructing evolutionary adaptive paths for protein engineering / Megan F. Cole ... [et al.]
  • Oligonucleotide recombination enabled site-specific mutagenesis in bacteria / Bryan M. Swingle
  • FX cloning : a versatile high-throughput cloning system for characterization of enzyme variants / Eric R. Geertsma
  • Use of Sulfolobus solfataricus PCNA subunit proteins to direct the assembly of multimeric enzyme complexes / Hidehiko Hirakawa and Teruyuki Nagamune
  • Gene synthesis by assembly of deoxyuridine-containing oligonucleotides / Romualdas Vaisvila and Jurate Bitinaite
  • Protein engineering : single or multiple site-directed mutagenesis / Pei-Chung Hsieh and Romualdas Vaisvila
  • Gene assembly and combinatorial libraries in S. cerevisiae via reiterative recombination / Nili Ostrov, Laura M. Wingler, and Virginia W. Cornish
  • Promiscuity-based enzyme selection for rational directed evolution experiments / Sandeep Chakraborty ... [et al.]
  • Rational protein sequence diversification by multi-codon scanning mutagenesis / Jia Liu and T. Ashton Cropp
  • Screening libraries for improved solubility : using E. coli dihydrofolate reductase as a reporter / Jian-Wei Liu and David L. Ollis
  • In vitro directed evolution of enzymes expressed by E. coli in microtiter plates / Bradley J. Stevenson, Sylvia H.-C. Yip, and David L. Ollis.
Samuelson, James C., 1968- Editor
Methods in molecular biology ; 978
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; v. 978.
Springer protocols (Series)

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