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Antigen processing : methods and protocols / edited by Peter van Endert.



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Publication date:
New York : Humana Press, c2013.
  • Book
  • xvii, 589 p. : ill. (some col.) ; 26 cm.
Includes bibliographical references and index.
  • Purification of large cytosolic proteases for in vitro assays: 20S and 26S proteasomes
  • Analysis of proteasome generated antigenic peptides by mass spectrometry
  • Using protease inhibitors in antigen presentation assays
  • Kinetic studies of cytoplasmic antigen processing and production of MHC class I ligands
  • Assaying peptide translocation by the peptide transporter TAP
  • In vitro reconstitution of the MHC class I peptide-loading complex
  • Studying mhc class i peptide loading and exchange in vitro
  • Measuring synthesis and degradation of MHC class I molecules
  • Studying ubiquitination of MHC class I molecules
  • Evaluation of viral interference with MHC class I-restricted antigen processing and presentation using a flow cytometry-based approach
  • Determining the activity of the transporter associated with antigen processing in the compartments of the secretory pathway
  • Biochemical large-scale identification of MHC class I ligands
  • Establishing MHC class I peptide motifs
  • Quantitating MHC class I ligand production and presentation using TCR-like antibodies
  • Biochemical analysis of naturally processed antigenic peptides presented by MHC class I molecules
  • Identifying source proteins for MHC class I-presented peptides
  • Purification, preparation, and use of chaperone-peptide complexes for tumor immunotherapy
  • Recombinant poxviruses: Versatile tools for immunological assays
  • Bioinformatics identification of antigenic peptide: Predicting the specificity of major MHC class I and II pathway players
  • Evaluating CD8(+) T cell responses in vitro
  • Cloning CD8(+) cytolytic T lymphocytes
  • Production of CD4(+) and CD8 (+) T cell hybridomas
  • Tracking antigen-specific CD8(+) T cells using MHC class I multimers
  • The purification of large numbers of antigen presenting dendritic cells from mouse spleen
  • Preparation of dendritic cells by in vitro cultures
  • Monitoring dendritic cell activation and maturation
  • Monitoring the intracellular routing of internalized antigens by immunofluorescence microscopy
  • Isolation of a specialized, antigen-loaded early endosomal subpopulation by flow cytometry
  • Preparing antigens suitable for cross-presentation assays in vitro and in vivo
  • Gene transduction in human monocyte-derived dendritic cells using lentiviral vectors
  • Pulse-chase analysis for studies of MHC class II biosynthesis, maturation, and peptide loading
  • Assembly of matched [alpha/beta] subunits to HLA class II peptide receptors
  • Studying MHC class II peptide loading and editing in vitro
  • Study of the allogeneic response induced by endothelial cells expressing hla class ii after lentiviral transduction
  • Antigen processing for mhc presentation via macroautophagy
  • Studying MHC class II transport in dendritic cells
  • In vitro digestion with proteases producing MHC class II ligands
  • MHC-II ubiquitination
  • Studying MHC class II presentation of immobilized antigen by B lymphocytes
  • Production of primary human CD4(+) T cell lines and clones
  • Analyzing antigen recognition by natural killer T cells
  • Chromatofocusing purification of CD1B-antigen complexes and their analysis by isoelectric focusing.
Endert, Peter van.
Methods in molecular biology, 1064-3745 ; 960
Springer protocols, 1949-2448
Methods in molecular biology (Clifton, N.J.) ; v. 960.
Springer protocols (Series)

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