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JAK-STAT signalling : methods and protocols / edited by Sandra E. Nicholson and Nicos A. Nicola.

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Language:
English.
Publication date:
2013
Imprint:
New York : Humana Press, c2013.
Format:
  • Book
  • xi, 320 p. : ill. (some col.) ; 26 cm.
Bibliography:
Includes bibliographical references and index.
Contents:
  • Analysis of Janus tyrosine kinase phosphorylation and activation / Jeremy A. Ross, Georgialina Rodriguez, and Robert A. Kirken
  • Co-immunoprecipitation protocol to investigate cytokine receptor-associated proteins, e.g., Janus kinases or other associated signaling proteins / Claude Haan and Serge Haan
  • In vitro JAK kinase activity and inhibition assays / Jeffrey J. Babon and James M. Murphy
  • Quantitative analysis of JAK binding using isothermal titration calorimetry and surface plasmon resonance / Jeffrey J. Babon
  • Determination of protein turnover rates in the JAK/STAT pathway using a radioactive pulse-chase approach / Anna Dittrich, Elmar Siewert, and Fred Schaper
  • Designing RNAi screens to identify JAK/STAT pathway components / Katherine H. Fisher, Stephen Brown, and Martin P. Zeidler
  • The use of JAK-specific inhibitors as chemical biology tools / Christopher J. Burns, David Segal, and Andrew F. Wilks
  • Methods for detecting mutations in the human JAK2 gene / Anthony J. Bench, E. Joanna Baxter, and Anthony R. Green
  • Detection of activated STAT proteins / Jean-Patrick Parisien and Curt M. Horvath
  • Detection of activated STAT species using electrophoretic mobility shift assay (EMSA) and potential pitfalls arising from the use of detergents / Serge Haan and Claude Haan
  • Flow cytometric analysis of STAT phosphorylation / Jane Murphy and Gabrielle L. Goldberg
  • Acetylation of endogenous STAT proteins / Torsten Ginter, Thorsten Heinzel, and Oliver H. Krämer
  • Detection and cellular localization of phospho-STAT2 in the central nervous system by immunohistochemical staining / Reza Khorooshi and Trevor Owens
  • Nuclear trafficking of STAT proteins visualized by live cell imaging / Velasco Cimica and Nancy C. Reich
  • Characterization of STAT self-association by analytical ultracentrifugation / Nikola Wenta and Uwe Vinkemeier
  • Constitutively active STAT5 constructs / Lynn M. Heltemes-Harris and Michael A. Farrar
  • Analysis of suppressor of cytokine signalling (SOCS) gene expression by real-time quantitative PCR / Tatiana B. Kolesnik and Sandra E. Nicholson
  • Detection of endogenous SOCS1 and SOCS3 proteins by immunoprecipitation and western blot analysis / Jian-Guo Zhang and Sandra E. Nicholson
  • In vitro ubiquitination and cytokine signaling components / Jeffrey J. Babon, Artem Laktyushin, and Nadia J. Kershaw
  • Production and crystallization of recombinant JAK proteins / Isabelle S. Lucet and Rebecca Bamert
  • Bacterial expression, purification, and crystallization of tyrosine phosphorylated STAT proteins / Florence Baudin and Christoph W. Müller.
Contributor:
Nicholson, Sandra E.
Nicola, Nicos, 1950-
Series:
Methods in molecular biology, 1064-3745 ; 967
Springer protocols
Methods in molecular biology (Clifton, N.J.) ; 967.
Springer protocols (Series)
Subjects:
ISBN:
9781627032414
162703241X
9781627032421
1627032428

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