Mass spectrometry of glycoproteins : methods and protocols
QH506 .M45 V.951
- Unknown QH506 .M45 V.951
- Includes bibliographical references and index.
- Introduction to glycosylation and mass spectrometry / Steven M. Patrie, Michael J. Roth, and Jennifer J. Kohler
- PART I ENRICHMENT AND ISOLATION METHODS
- Tandem lectin weak affinity chromatography for glycoprotein enrichment / Zhi Yuan Ma, Yuliya Skorobogatko, and Keith Vosseller
- CSC technology: Selective labeling of glycoproteins by mild oxidation to phenotype cells / Andreas Hofmann, Damaris Bausch-Fluck, and Bernd Wollscheid
- Use of boronic acid nanoparticles in glycoprotein enrichment / Yawei Xu, Lijuan Zhang, and Haojie Lu
- Incorporation of unnatural sugars for the identification of glycoproteins / Balyn W. Zaro, Howard C. Hang, and Matthew R. Pratt
- Characterization of membrane-associated glycoproteins using lectin affinity chromatography and mass spectrometry / Yashu Liu, Jintang He, and David M. Lubman
- Sialic acid capture-and-release and LC-MS(n) analysis of glycopeptides / Jonas Nilsson and Göran Larson
- PART II SAMPLE PREPARATION
- In-solution digestion of glycoproteins for glycopeptide-based mass analysis / Eden P. Go, Kathryn R. Rebecchi, and Heather Desaire
- Nano-HPLC-MS of glycopeptides obtained after nonspecific proteolysis / Gerhild Zauner, Carolien A.M. Koeleman, André M. Deelder, and Manfred Wuhrer
- PART III SEPARATION METHODS
- Glycopeptide enrichment for MALDI-TOF mass spectrometry analysis by hydrophilic interaction liquid chromatography solid phase extraction (HILIC SPE) / Pia Hønnerup Jensen, Simon Mysling, Peter Højrup, and Ole Nørregaard Jensen
- Separation and identification of glycoforms by capillary electrophoresis with electrospray ionization mass spectrometric detection / Alina D. Zamfir, Corina Flangea, Alina Serb, Ana-Maria Zagrean, Andreas M. Rizzi, and Eugen Sisu
- Structural separations by ion mobility-MS for glycomics and glycoproteomics / Larissa S. Fenn and John A. McLean
- PART IV QUANTITATION METHODS
- Quantitative analysis of glycoprotein glycans / Ron Orlando
- Stable isotope labeling of N-glycosylated peptides by enzymatic deglycosylation for mass spectrometry-based glycoproteomics / Hiroyuki Kaji and Toshiaki Isobe
- Approaches for site mapping and quantification of o-linked glycopeptides / Peng Zhao, Stephanie H. Stalnaker, and Lance Wells
- Glycan profiling: Label-free analysis of glycoproteins / Yoshinao Wada
- PART V COMPUTATIONAL TOOLS
- Introduction to informatics in glycoprotein analysis / Kiyoko F. Aoki-Kinoshita
- Software tools for glycan profiling / Chuan-Yih Yu, Anoop Mayampurath, and Haixu Tang
- PART VI CASE STUDIES IN MASS SPECTROMETRY OF GLYCOPROTEINS
- Quantitative characterization of glycoproteins in neurodegenerative disorders using iTRAQ / Min Shi, Hyejin Hwang, and Jing Zhang
- Quantitative proteomic analysis of N-linked glycoproteins in human tear fluid / Lei Zhou and Roger W. Beuerman
- Elucidation of N-glycosites within human plasma glycoproteins for cancer biomarker discovery / Penelope Drake, Birgit Schilling, Brad Gibson, and Susan Fisher
- Characterizing the glycosylation state of therapeutic recombinant glycoproteins. / Nicole Samuels, David Kates, Jun Liu, and Joanne Severs.
- Publisher's Summary
- Glycosylation is the most abundant post-translational modification of proteins. Estimates vary widely, but a common assessment is that upwards of 50% of eukaryotic proteins are modified by some type of glycan. In Mass Spectrometry of Glycoproteins: Methods and Protocols, expert researchers in the field detail many of the methods that are now commonly used for glycoproteomics. These methods and techniques include robust sample preparation techniques; advanced chromatographic strategies for improving dynamic range; state-of-the-art mass spectrometry instrumentation and associated ionization and fragmentation methods; and informatics tools used for identifying glycoproteins and characterizing the associated glycans. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry of Glycoproteins: Methods and Protocol is an essential resource for those who work at the interface of glycobiology and mass spectrometry.
(source: Nielsen Book Data)
- Publication date
- edited by Jennifer J. Kohler, Steven M. Patrie.
- Methods in molecular biology, 1064-3745 ; 951
- Springer Protocols
- Also available in electronic format via the World Wide Web.